Destrehan High School’s football team is no longer undefeated.

But it was the Louisiana High School Athletic Association that handed the Wildcats’ their first loss of the season.

The LHSAA ruled Wednesday that Destrehan would have to forfeit Friday’s 49-24 victory over South Lafourche.

It was one of several stiff penalties the LHSAA gave Destrehan in the case that involved assistant coaches spying on South Lafourche’s football practices last week.

Five assistant coaches (Ryan Fournier, Lance Ledet, Daniel Luquet, Wayne Sentillis and Greg Boyne) were all suspended. Fournier can’t coach in any games for the rest of the regular season and the playoffs. The other four are suspended for the rest of the regular season, but can return for the playoffs. But in the playoffs, they will only be allowed to coach from the press box.

Destrehan has two regular season games left. The Wildcats play H.L. Bourgeois on Friday and East St. John next week.

The school was also fined $1,000.

Destrehan, which was 8-0 and ranked No. 3 in The New Orleans Advocate’s large school rankings, is now 7-1 and 4-1 in District 7-5A. The Wildcats were the No. 1-seeded team in Class 5A according to the LHSAA’s latest power rankings,

The investigation involves Hudl, a video application that allows teams to study themselves and scout opponents.

“It’s a film-sharing program, and they were on a part that they weren’t supposed to be on,” LHSAA Executive Director Kenny Henderson said Tuesday night.

Destrehan coach Stephen Robicheaux couldn’t be reached for comment, but he did release a statement, emailed by Stevie Crovetto, the St. Charles Parish Public Schools director of public information.

“I am disappointed that this happened and I respect the LHSAA’s decision,” the statement said. “My concern is about our kids in this unfortunate situation. My focus is to address the needs of our players and move on from this.”

South Lafourche is now 5-3 and 4-1 in district play. South Lafourche plays Thibodaux on Thursday.